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Programme: Biotechnology and Biological Sciences Research Council (BBSRC), UK

Project title: Resistance evaluation to Phytophthora infestans of Solanum nigrum population used for mapping and isolation of R genes

Contract nr: 587/N-BBSRC/2009/0


Project duration: 14.12.2009 – 13.04.2012

Main coordinator: Jonathan Jones, The Sainsbury Laboratory (TSL), Norwich, United Kingdom

Polish partner: Plant Breeding and Acclimatization Institute, National Research Institute, Młochow Research Division - prof. Ewa Zimnoch-Guzowska, Department of Potato Genetics and Parental Lines, Jadwiga Sliwka, PhD, Laboratory of Potato Pathology

Main objectives:
The goal of the project carried out in collaboration with prof. Jonathan Jones of the TSL was to improve the knowledge on the structure, evolution and function of R genes encoding resistance to Phytophthora infestans, the pathogen causing potato blight, and to obtain transgenic potato forms resistant, in a permanent manner, to the disease. It was planned to clone new genes for resistance to potato late blight using a novel approach. It is based on a huge efficiency of the sequencing device Illumina GA2, which is owned by TSL. The activities carried out in IHAR-PIB Młochów Research Division consisted of preparation of plant material – the source of resistance genes. Within the project, we obtained lines of F4 generation from three crosses between resistant and susceptible to potato late blight Solanum nigrum genotypes. On the basis of the results obtained in laboratory detached leaf assays, we identified homozygous resistant and susceptible lines. Their seed were delivered to TSL, where the work on cloning resistance genes is in progress.

Link: http://www.bbsrc.ac.uk/organisation/institutes/institutes-of-bbsrc/institutes-of-bbsrc-index.aspx

Contact: e.zimnoch-guzowska@ihar.edu.pl; j.sliwka@ihar.edu.pl

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