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Department of Functional Genomics

Department of Functional Genomics

Professor Anna Nadolska-Orczyk, Ph.D., D.Sc.
phone: (+48 22) 7334619
e-mail: a.orczyk@ihar.edu.pl


Our research is focused on application of genetic transformation via Agrobacterium tumefaciens and RNAi technology of cereals (wheat, triticale, oat) and pea cultivars for analysis of gene function and obtaining breeding lines with improved characteristics.


Research projects:


1. Agrobacterium-mediated transformation of cereals: wheat (Triticum aestivum L.), triticale (Triticale), oat (Avena sativa L.) and barley (Hordeum vulgare L.):

·         adaptation of genetic transformation methods developed in the lab to polish breeding lines and cultivars,

·         analysis of factors influencing expression and silencing of transgenes in the listed species.

2. Application of RNAi in functional genetics and biotechnology of cereals and pea:          

·         analysis of influence of RNAi process on the level of expression and silencing of native genes in wheat, triticale, barley and pea,

·         silencing of  Pina i Pinb genes in wheat and triticale by RNAi method and analysis of grain texture,

·         functional analysis of CKX gene family in regulation of cytokinin level in barley and wheat and its influence on phenotype modification.

3. Identification of molecular-biochemical-physiological markers/factors of productivity, root system and determinants of grain texture in cereals.


Selected publications:

·         Zalewski W, Orczyk W, Gasparis S, Nadolska-Orczyk A. 2012. HvCKX2gene silencing by biolistic or Agrobacterium-mediated transformation in barley leads to different phenotypes. BMC Plant Biology, 12:206; http://www.biomedcentral.com/1471-2229/12/206

·         Dmochowska-BogutaM., Nadolska-Orczyk A., Orczyk W. 2012. Roles of peroxidases and NADPH oxidases in the oxidative response of wheat (Triticum aestivum) to brown rust (Puccinia triticina) infection. Plant Pathol. Doi: 10.1111/ppa.12009

·         Binka A., Orczyk W., Nadolska-Orczyk A. 2012. The Agrobacterium-mediated transformation of common wheat (Triticum aestivum L.) and triticale (x Triticosecale Wittmack): role of the binary vector systems and selection cassettes. J. Appl. Genet. 2012. 53:1-8. DOI: 10.1007/s13353-011-0064-y.

·         Kawałek A, Dmochowska-Boguta M, Nadolska-Orczyk A, Orczyk W. 2012. Anew BSMV-based vector with modified β molecule allows simultaneous and stable silencing of two genes. Cell. Mol.Biol. Lett. 17: 107-123. DOI: 10.2478/s11658-011-0041-9, 17: 107-123.

·         Gasparis S., Orczyk W., Zalewski W., Nadolska-Orczyk A. 2011. The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness. J. Exp. Bot. 62 (11): 4025-4036. DOI:10.1093/jxb/err103

·         Zalewski W., Galuszka P., Gasparis S., Orczyk W., Nadolska-Orczyk A. 2010. Silencing of the HvCKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity. J. Exp. Bot. 61: 1839-1851. DOI:10.1093/jxb/erq052

·         Nadolska-Orczyk A., Galuszka P., Zalewski W., Orczyk W. 2010. ADNA cassette, binary vector, and strain of A. tumefaciens and a method of producing cereal plant of increased productivity and/or root mass. International patent application No. PCT/PL2010/050019

·         Orczyk W., Dmochowska-Boguta M., Czembor H.J., Nadolska-Orczyk A. 2010. Spatiotemporal patterns of oxidative burst and micronecrosis in resistance of wheat to brown rust infection. Plant Pathol. 59: 567-575. DOI: 10.1111/j.1365-3059.2010.02257.x

·         Nadolska-Orczyk A., Gasparis S., Orczyk W. 2009. The determinants of grain texture in cereals. J. Appl. Genet. 50: 185-197.

·         Gasparis S., Bregier C., Orczyk W., Nadolska-Orczyk A. 2008. Agrobacterium-mediated transformation of oat (Avena sativa L) cultivars via immature embryo and leaf explants. Plant Cell Rep. 27: 1721-1729. DOI: 10.1007/s00299-008-0593-y

·         Nadolska-Orczyk A. 2008. Agrobacterium-mediated transformation of pea (Pisum sativum L.). In: Handbook of a New Technologies for Genetic Improvement of Legumes. (Ed. P.B. Kirti); CRC Press, New York, str. 187-202.

·         Nadolska-Orczyk A., Pietrusinska A., Binka-Wyrwa A., Kuc D, Orczyk W. 2007. Diploid potato (Solanum tuberosum L.) as a model crop to study transgene expression. Cell. Mol. Biol. Lett. 12: 206-219.

·         Przetakiewicz J., Nadolska-Orczyk A., Kuc D., Orczyk W. 2007. Tetraploid somatic hybrids of potato (Solanum tuberosumL.) obtained from diploid breeding lines. Cell. Mol. Biol. Lett. 12: 253-267.

·         Nadolska-Orczyk A., Przetakiewicz A., Kopera K., Bińka A., Orczyk W. 2005. Efficient method of Agrobacterium-mediated transformation for triticale (x Triticosecale Wittmack). J. Plant Growth Reg. 24: 2-10.

·         Przetakiewicz A., Karaś A., Orczyk W., Nadolska-Orczyk A. 2004. Agrobacterium-mediated transformation of polyploid cereals. The efficiency of selection and transgene expression in wheat. Cell. Mol. Biol. Lett. 9: 903-917.

·         Nadolska-Orczyk A., Orczyk W. 2003. Agrobacterium-mediated transformation of cereals. In: Plant Genetic Engineering Vol 2: Improvement of Food Crops. (Ed. P.K. Jaiwal and R.P. Singh); Sci. Tech. Publishing LLC, USA, pp. 1-25.

·         Przetakiewicz A., Orczyk W., Nadolska-Orczyk A. 2003. The effect of auxin on plant regeneration of wheat, barley and triticale. Plant Cell, Tissue Org. Cult. 73: 245-256.

·         Orczyk W., Przetakiewicz J., Nadolska-Orczyk A. 2003. Somatic hybrids of Solanum tuberosum – application to genetics and breeding. Plant Cell, Tissue Org. Cult. 73: 245-256.

·         Przetakiewicz J., Nadolska-Orczyk A., Orczyk W. 2002. The Use of RAPD and Semi-random Markers to Screen Somatic Hybrids Between Diploid Lines of Solanum tuberosum L. Cell. Mol. Biol. Lett. 7: 6711-676.

·         Rhodes D., Nadolska-Orczyk A., Rich P.I. 2002. Salinity, osmolytes and compatible solutes. In: Salinity: Environment – Plant – Molecules. (Eds. A. Läuchli and U. Lüttge), Kluwer Academic, Boston, N.Y., pp. 181-204.

·         Rhodes D., Nadolska-Orczyk A. 2001. Plant Stress Physiology. In: Encyclopedia of Life Sciences. pp 1-7, (http://www.els.net/) Macmillan Publishers Ltd. Registered No. 785998. England.

·         Nadolska-Orczyk A., Orczyk W. 2000. Study of the factors influencing Agrobacterium-mediated transformation of pea (Pisum sativum L.). Mol. Breed. 6: 185-194.

·         Nadolska-Orczyk A., Orczyk W., Przetakiewicz A. 2000. Agrobacterium-mediated transformation of cereals – from technique development to its application. Acta Physiol. Plant. 22: 77-88.

·         Nadolska-Orczyk A. 2000. Invitro plant regeneration and genetic transformation of grain legumes. (Polish, Eng. Sum.) Monographs and Scientific Dissertations of PBAI. 2: 1-63. 

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