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Department of Potato Genetics and Parental Lines

Professor Waldemar Marczewski, D. Sc.
phone: (+48 22) 729 90 89 or 729 92 48 ext. 215
e-mail: w.marczewski@ihar.edu.pl


Laboratory of Potato Pathogens (go up)

Professor Jadwiga Śliwka, D.Sc.
phone: (+48 22) 729 90 89 ext. 219
e-mail: j.sliwka@ihar.edu.pl

M sc Marta Brylińska, M Sc Sylwester Sobkowiak, M Sc Emil Stefańczyk

 

Objectives:

  • Monitoring of Phytophthora infestans population in Poland: characterization of P. infestansisolates for virulence, mating type, resistance to metalaxyl, mitochondrial DNA haplotype, genotype using SSR markers


Identification of mating type of Phytophthora infestans isolate.

  • Collection and maintenance of fungal potato pathogens
  • Identification and diversity study on fungi of the Fusariumgenus associated with dry rot of potato tubers


Collection of Phytophthora inferstans.

  • Tests for potato resistance to P. infestans, Fusarium,

A list and descriptions of P. infestans isolates in IHAR Młochów collection.  IHAR Młochów is the owner of the presented data and they cannot be published without the owner’s permission. The isolates are freely available for noncommercial purposes according to the material transfer agreement.

Click here to download the file.


Potato tubers infected with Phytophthora infestans on 1-9 scale.

  • Marker Assisted Selection (MAS) of potato resistant to P. infestanspossessing Rpi-phul, Rpi-mch1and Rpi-rzc1genes
  • Characterization of sources of resistance to P. infestans
  • Mapping and pyramiding potato Rgenes for P. infestans resistance, study on Rpi-phu1expression


Symptoms of infection of susceptible and resistant black nightshade (Solanum nigrum)
to Phytophthora infestans.

 


 Publications 2010-2014:

1.        Śliwka J., Jakuczun H., Kamiński P., Zimnoch-Guzowska E. 2010. Marker-assisted selection of diploid and tetraploid potatoes carrying Rpi-phu1, a major gene for resistance to Phytophthora infestans. J. Appl. Genet. 51(2): 133–140.

2.        Chmielarz M., Sobkowiak S., Lebecka R., Śliwka J. 2010. Chosen characteristics of Polish Phytophthora infestans isolates. PPO Special Reports 14: 39-44.

3.        Tomczyńska I., Śliwka J. 2011. Piramidyzacja genów odporności w roślinach uprawnych. Biul.IHAR 262: 77-88.

4.        Świątek M., Śliwka J. 2011. Przegląd badań nad regulacją ekspresji genów głównych odporności roślin na patogeny. Biuletyn IHAR 262: 89-101.

5.        Sobkowiak S., Śliwka J., Chmielarz M., Lebecka R., Zimnoch-Guzowska E. 2011. Resistance to metalaxyl of Phytophthora infestans isolates occurring in Poland in 2006-2010. Phytopathologia 61: 29-35.

6.        Śliwka J., Jakuczun H., Chmielarz M., Hara-Skrzypiec A., Tomczyńska I., Kilian A, Zimnoch-Guzowska E. 2012. A new resistance gene against potato late blight originating from Solanum x michoacanum maps to potato chromosome VII. Theor. Appl. Genet. 124:397–406.

7.        Śliwka J., Jakuczun H., Chmielarz M., Hara-Skrzypiec A., Tomczyńska I., Kilian A., Zimnoch-Guzowska E. 2012.  Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour. BMC Genet. 13 (1): 11

8.        Sobkowiak S., Zarzycka H., Śliwka J. 2012. The influence of long-term storage in liquid nitrogen on survival and pathogenicity of Phytophthora infestans isolates. J. Plant Prot. Res. 52: 479-485.

9.        Śliwka J., Tomczyńska I., Chmielarz M., Stefańczyk E., Lebecka R., Zimnoch-Guzowska E. 2012. Resistance to Phytophthora infestans in three Solanum nigrum F3 families. Plant Breed. Seed Sci. 66: 63-73.

10.      Stefańczyk E, Śliwka J. 2013 Wpływ fotoperiodu na biologię ziemniaka. Biuletyn IHAR 267:57-69

11.      Śliwka J., Zimnoch-Guzowska E. 2013. Resistance to late blight in potato. In: Tuberosa R, Varshney RK (ed) Translational Genomics for Crop Breeding, Volume 1 - Biotic Stress. USA, Wiley-Blackwell Publishers, 221-240

12.      Smyda P., Jakuczun H., Dębski K., Śliwka J., Thieme R., Nachtigall M., Wasilewicz-Flis I., Zimnoch - Guzowska E. 2013. Development of somatic hybrids Solanum x michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. michoacanum plants as potential sources of late blight resistance for potato breeding. Plant Cell Rep. 32: 1231-1241, DOI 10.1007/s00299-013-1422-5

13.      Śliwka J., Świątek M., Tomczyńska I., Stefańczyk E., Chmielarz M., Zimnoch-Guzowska E. 2013. Influence of genetic background and plant’s age on expression of potato late blight resistance gene Rpi-phu1 during incompatible interaction with pathogen. Plant Pathology, 62: 1072–1080, Doi: 10.1111/ppa.12018

14.      Jupe F, Witek K, Verweij W, Śliwka J, Pritchard L, Etherington GJ, Maclean D, Cock PJ, Leggett RM, Bryan GJ, Cardle L, Hein I, Jones JD. 2013 Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations. Plant J. 76(3):530-544. doi: 10.1111/tpj.12307

15.      Lebecka R, Sobkowiak S. 2013 Host-pathogen interaction between Phytophthora infestans and Solanum tuberosum following exposure to short and long daylight hours. Acta Physiologiae Plantarum. 35:1131-1139

16.      Tarwacka J, Polkowska-Kowalczyk L, Kolano B, Śliwka J, Wielgat B. 2013 Interspecific somatic hybrids Solanum villosum (+) S. tuberosum, resistant to Phytophthora infestans. J Plant Physiol. 170(17):1541-8. doi: 10.1016/j.jplph.2013.06.013.

17.      Chmielarz M, Sobkowiak S, Dębski K, Cooke DEL, Brurberg MB, Śliwka J. 2014 Diversity of Phytophthora infestans from Poland. Plant Pathology 63: 203-211 DOI:10.1111/ppa.12076

18.      Tomczyńska I, Stefańczyk E, Chmielarz M, Karasiewicz B, Kamiński P, Jones JDG, Lees AK, Śliwka J. 2014 A locus conferring effective late blight resistance in potato cultivar Sárpo Mira maps to chromosome XI. Theor Appl Genet 127: 647-657 DOI: 10.1007/s00122-013-2248-9

19.      Tomczyńska I, Jupe F, Hein I, Marczewski W, Śliwka J. 2014 Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX. Molecular Breeding 34:471-480 DOI: 10.1007/s11032-014-0050-2

20.      Nilsson RH, Hyde KD, Pawlowska J, Ryberg M, Tedersoo L, Aas AB, Alias SA, Alves A, Anderson CL, Antonelli A, Arnold AE, Bahnmann B, Bahram M, Bengtsson-Palme J, Berlin A, Branco S, Chomnunti P, Dissanayake A, Drenkhan R, Friberg H, Frøslev TG, Halwachs B, Hartmann M, Henricot B, Jayawardena R, Jumpponen A, Kauserud H, Koskela S, Kulik T, Liimatainen K, Lindahl B, Lindner D, Liu J-K, Maharachchikumbura S, Manamgoda D, Martinsson S, Neves MA, Niskanen T, Nylinder S, Pereira OL, Pinho DB, Porter TM, Queloz V, Riit T, Sanchez-García M, de Sousa F, Stefańczyk E, Tadych M, Takamatsu S, Tian Q, Udayanga D, Unterseher M, Wang Z, Wikee S, Yan J, Larsson E, Larsson K-H, Kõljalg U, Abarenkov K. 2014 Improving ITS sequence data for identification of plant pathogenic fungi. Fungal Diversity 67:11-19

21.      Hietala AM, Stefańczyk E, Nagy NE, Fossdal CG, Alfredsen G. 2014 Influence of wood durability on the suppressive effect of increased temperature on wood decay by the brown-rot fungus Postia placenta. Holzforschung 68: 123-131

22.      Brylińska M., Śliwka J. 2014. Efektory – kluczowe białka w interakcji ziemniak – Phytophthora infestans. Biuletyn IHAR 271:109-118


Laboratory of Biotechnology (go up)

Professor Waldemar Marczewski, D.Sc.
phone: (+48 22) 729 92 48 ext. 215
e-mail: w.marczewski@ihar.edu.pl

Ph D Dorota Sołtys-Kalina, Ph D Katarzyna Szajko, M Sc Iwona Wasilewicz-Flis, Ph D Agnieszka Hara-Skrzypiec, Ph D Paulina Smyda-Dajmund, Eng. Małgorzata Osińska

 

Objectives:

  • Mapping potato for genes of resistance to pathogens,
  • Genetics and molecular studies of  potato response to biotic and abiotic stress
  • Identification of DNA markers useful to marker-assisted selection in potato breeding.
  • Genetic analysis of new sources of quality traits in diploid potato
  • Preservation and characteristics of potato genetic resources in field collection
  • Exploitation of diploid biodiversity in development of cultivated potato S. tuberosum with use of 4x-2x crosses
  • Somatic hybridization - phenotypic and  genotypic characteristics of somatic hybrids

 

Examples of the research results:


Response of cvs. Albatros, Neptun, Romula, Rywal and Sekwana plants to infection with
PVYNTNBo: detached leaves incubated at 20°C or 28°C five days after infection.


Positions of the loci Ny-1 on potato chromosome IX in cvs. Rywal, Albatros and Sekwana
and Ny-2 on chromosome XI in cv. Romula.


The SC8951139 and S1d11390 markers linked to the Ny-1 genesin cvs Rywal and Albatros
and the B11.61600 marker linked to the Ny-2 gene in cv Romula.


Evaluation of blackspot bruise – using of drum test


Evaluation of chipping ability


Filed collection of diploid hybrids

 


Publications (2010 – 2014):

1.    Staniaszek M, Szajko K, Uliński Z, Szczech M, Marczewski W (2010) BseGI Restriction of the Polymerase Chain Reaction Amplicon Th444 Is Required to Distinguish Biotypes of Trichoderma aggressivum Causing Serious Losses in Mushroom (Agaricus bisporus) Production. Hortscience 45(12):1910–1911

2.    Krusiewicz D, Jakuczun H, Wasilewicz-Flis I, Strzelczyk-Żyta D, Marczewski W (2011) Molecular mapping of the AOX1a and β-AmyI genes in potato. Plant Breeding 130, 500—502

3.    Staniaszek M, Szajko K, Kozik EU, Nowakowska M, Marczewski W (2012) The novel ps and ps-2 specific markers for selection of functional male sterile tomato lines in breeding programs and hybrids seed production. Journal of Agricultural Science 4 (10): 61-67

4.    Soltys D, Rudzinńska-Langwald A, Gniazdowska A, Wiśniewska A, Bogatek R (2012) Inhibition of tomato (Solanum lycopersicum L.) root growth by cyanamide is due to altered cell division, phytohormone balance and expansin gene expression. Planta 236: 1629-1638

5.    Soltys D, Gniazdowska A, Bogatek R (2012) Phytotoxic effects of cyanamide on seed germination and seedling growth of weed and crop species. Acta Biologica Cracoviensia Series Botanica 54(2)

6.    Hara-Skrzypiec A. Jakuczun H. (2013) Diploid Potato Hybrids as Sources of Resistance to Blackspot Bruising. American Journal of Potato Research 90: 451-459

7.    Pieczyński M, Marczewski W, Hennig J, Dolata J, Bielewicz D, Piontek P, Wyrzykowska A, Krusiewicz D, Strzelczyk-Zyta D, Konopka-Postupolska D, Krzesłowska M, Jarmołowski A, Szweykowska-Kulińska Z (2013) Downregulation of CBP80 gene expression as a strategy to engineer a drought-tolerant potato. Plant Biotechnology Journal 11: 459-469

8.    Smyda P., Jakuczun H., Dębski K., Śliwka J., Thieme R., Nachtigall M., Wasilewicz-Flis I., Zimnoch-Guzowska E. (2013). Development of somatic hybrids Solanum x michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. x michoacanum plants as potential sources of late blight resistance for potato breeding. Plant Cell Reports 32: 1231-1241

9.    Soltys D, Krasuska U, Bogatek R, Gniazdowska A (2013) Allelochemicals as Bioherbicides — Present and Perspectives [w]: Herbicides - Current Research and Case Studies in Use pod red. Andrew J. Pirce i Jessica Kelton. Wydawnictwo InTech, Chorwacja: 2013; 517-542. ISBN 978-953-51-1112-2

10.  Sołtys D (2013) Solanina i chaconina – główne glikoalkaloidy ziemniaka uprawnego (Solanum tuberosum L.). Kosmos 62(1): 129-138

11.  Soltys D, Gniazdowska A, Bogatek R (2013) Inhibition of tomato (Solanum lycopersicum L.) root growth by cyanamide is not always accompanied with enhancement of ROS production. Plant Signaling and Behavior 8(5): e23994-(1-2)

12.  Baebler S, Witek K, Petek M, Stare K, Tušek Žnidarič M, Pompe Novak M, Renaut J, Szajko K, Strzelczyk-Zyta D, Marczewski W, Morgiewicz K, Gruden K, Hennig J (2014) Salicylic acid is an indispensible component of Ny-1 resistance gene-mediated response against Potato virus Y infection in potato. Journal of Experimental Botany 65 (4): 1095-1109

13.  Szajko K, Strzelczyk-Żyta D, Marczewski W (2014) Ny-1 and Ny-2 genes conferring hypersensitive response to potato virus Y (PVY) in cultivated potatoes: mapping and marker-assisted selection validation for PVY resistance in potato breeding. Molecular Breeding 34 (1): 267-271

14.  Soltys D, Rudzińska-Langwald A, Kurek W, Szajko K, Sliwinska E, Bogatek R, Gniazdowska A (2014) Phytotoxic cyanamide affects maize (Zea mays L.) root growth and root tip function: from structure to gene expression. Journal of Plant Physiology 171(8):565-75

15.  Tomczyńska I, Jupe F, Hein I, Marczewski W, Śliwka J (2014) Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX. Molecular Breeding 34 (2): 471-480

 

Patents:

1.    Marczewski W, Szajko K. 2013 patent nr. P.396680 „Sposób identyfikacji molekularnej genów Ny-1A, Ny-1N i Ny-1S warunkujących odporność ziemniaka (Solanum tuberosum L.) na zakażenie wirusem Y ziemniaka (PVY, Potato virus Y)”.

2.    Marczewski W., Szajko K. 2014 patent nr. P 399117„Sposób identyfikacji molekularnej genu Ny-2, warunkującego reakcję nadwrażliwości ziemniaka (Solanum tuberosum L.) na zakażenie wirusem Y ziemniaka (PVY, Potato virus Y)”


Laboratory of Phytopathology (go up)

Renata Lebecka, D.Sc., Assoc. Prof. IHAR-PIB
phone: (+48 22) 729 92 48 w. 207
e-mail: r.lebecka@ihar.edu.pl

PhD Zhimin Yin, MSc Krystyna Michalak, MSc Anna Grupa

 

Objectives:

  • Studies on interactions between viruses, vectors and host plants in the process of infection and in the pathogenesis

 


Symptoms of infection with PVYN on Chenopodium album


Extremely severe reaction of Physalis floridana plants to infection with PVYNW


Detection of different strains of PVY using multiplex PCR

  • Resistance of potato breeding lines to PLRV
  • Diagnostics of viruses in breeding and experimental material
  • Characterization of strains of potato viruses,
  • Assessment of potato resistance to viruses,


  Symptoms of infection with PVYNTN

  • Evaluation of potato reaction to viral infection (PVY, PLRV, PVM, TRV),


Leaf necroses on variety sensitive to PVY - grafting test

  • Resistance to viruses of potato breeding lines
  • Collection and maintenance of viral potato pathogens
  • Evaluation of virus sources and test plants for identification and detection of  potato viruses


Evaluation of PVYo on Chenopodium ssp. leaves

  • Studies on viruses causing necrotic symptoms in potato tubers (TRV, PMTV),
  • Advisory and training activities for seed inspectors, advisors and farmers.

 


Publications 2009 -2014

1.    Nechwatal J., Lebecka R. 2014. Genetic and phenotypic analyses of Pythium isolates from reed suggest the occurrence of a new species, P. phragmiticola, and its involvement in the generation of a natural hybrid. Mycoscience 55 (2) 134-143

2.    Lebecka, R., S. Sobkowiak. 2013. Host-pathogen interaction between Phytophthora infestans and Solanum tuberosum following exposure to short and long daylight hours. Acta Physiologiae Plantarum 35:1131-1139

3.    Andrivon, D., J. Avendaño-Córcoles, A. M. Cameron, S. F. Carnegie, L. R. Cooke, R. Corbie`re, D. Detourne, L. J. Dowley, D. Evans, K. Forisekova, D. G. Griffin, A. Hannukkala, A. K. Lees, R. Lebecka, F. Niepold, Z. Polgar, D. S. Shaw, J. Thompson, B. Trognitz, H. M. G. van Raaij, E. Zimnoch-Guzowska, 2011. Stability and variability of virulence of Phytophthora infestans assessed in a ring test across European laboratories. Plant Pathology 60: 556-565

4.    Lebecka R. 2009. Inheritance of resistance in Solanum nigrum to Phytophthora infestansEuropean Journal of Plant Pathology 124: 345-348

5.    Yin Z, Pawełkowicz M, Michalak K, Chrzanowska M, Zimnoch-Guzowska E. 2014. Single nucleotide polymorphisms and reading frame shifts in RNA2 recombinant regions of tobacco rattle virus isolates Slu24 and Deb57. Archives of Virology, Ms. No. ARVI-D-14-00058R1

6.    Yin Z., Chrzanowska M., Michalak K., Zimnoch-Guzowska E. 2014. Alteration of host-encoded miRNAs in virus infected plants – experimentally verified. Rozdział książki Plant Virus-Host Interaction. Wydawca: RK Guar, Thomas Hohn and Pradeep Sharma. Elsevier Inc.. Rozdział 2. (Strony 17-55)

7.    Yin Z, Pawełkowicz M, Michalak K, Chrzanowska M, Zimnoch-Guzowska E. 2014. The genomic RNA1 and RNA2 sequences of the tobacco rattle virus isolates found in Polish potato fields. Virus Res. 185: 110-113

8.    Santala J, O. Samuilova, A. Hannukkala, S. Latvala, H. Kortemaa, U. Beuch, A. Kvarnheden, P. Persson, K. Topp, K. Ørstad, C. Spetz, S.L. Nielsen, H.G. Kirk, M. Budziszewska, P. Wieczorek, A. Obrępalska-Stęplowska, H. Pospieszny, A. Kryszczuk, J. Sztangret-Wiśniewska, Z. Yin, M. Chrzanowska, E. Zimnoch-Guzowska, E. Jackeviciene, L. Taluntytė, N. Pūpola, J. Mihailova, I. Lielmane, L. Järvekülg, K. Kotkas, E. Rogozina, A. Sozonov, I. Tikhonovich, P. Horn, I. Broer, S. Kuusiene, J. Staniulis, J.G. Uth, G. Adam, J.P.T. Valkonen, 2010. Detection, distribution and control of Potato mop-top virus, a soil-borne virus, in northern Europe. Annals of Applied Biology 157: 163-178

9.    Zimnoch-Guzowska E., Yin Z., Chrzanowska M., Flis B. 2013. Sources and effectiveness of potato PVY resistance in IHAR’s breeding research. American Journal of Potato Research 90: 21-27

10.  Yin Z., Chrzanowska M., Michalak K., Zagórska H., Zimnoch-Guzowska E. 2012. Recombinants of PVY strains predominate among isolates from potato crop in Poland. Journal of Plant Protection Research 52: 214-219

11.  Kaliciak A., Syller J. 2009. New hosts of Potato virus Y (PVY) among common wild plants in Europe. European Journal of Plant Pathology 124: 707-713

12.  Kozłowska-Makulska A., Beuve M., Syller J., Szyndel M.S., Lemaire O., Bouzoubaa S., Herrbach E. 2009. Aphid transmissibility of different European beet Polerovirus isolates. European Journal of Plant Pathology 125: 337-341

13.  Kaliciak A., Syller J. 2009. Przenoszenie różnych genetycznie izolatów wirusa Y ziemniaka przez mszyce i podatność chwastów na infekcję wirusem. Biuletyn IHAR nr 253: 285-295

14.  Garbaczewska G., Otulak K., Chouda M., Chrzanowska M. 2012. Ultrastructural studies of plasmodesmatal and vascular translocation of Tobacco rattle virus (TRV) in tobacco and potato. Acta Physiologiae Plantarum 34: 1229–1238 

15.  Otulak K., Chouda M., Chrzanowska M., Garbaczewska G. (2012). Ultrastructural effects of infection caused by Tabacco rattle virus transmitted by Trichodorus primitivus in potato and tobacco tissues. Canadian Journal of Plant Pathology34: 126-138

16.  Zoteyeva N., Chrzanowska M., Flis B., Zimnoch-Guzowska E. (2012). Resistance to pathogens of the potato accessions from the collection of N.I. Vavilov Institute of Plant Industry (VIR) American Journal of Potato Research 894: 277-293

17.  Syller J.2012. Facilitative and antagonistic interactions between plant viruses in mixed infections. Molecular Plant Pathology 13: 204-216

18.  Syller J., Grupa A. 2014. The effects of co-infection by different Potato virus Y (PVY) isolates on virus concentration in solanaceous hosts and efficiency of transmission. Plant Pathology 63: 466-475

19.  Syller J.2014. Biological and molecular events associated with simultaneous transmission of plant viruses by invertebrate and fungal vectors. Molecular Plant Pathology 15: 417-426


Laboratory of Parental Lines and Breeding Methods (go up)

Bogdan Flis, D.Sc., Assoc. Prof. IHAR-PIB
phone: (+48 22) 729 92 48 ext. 216
e-mail: b.flis@ihar.edu.pl

 

Professor Ewa Zimnoch-Guzowska D.Sc., Ph.D. Beata Tatarowska, Ph.D. Jarosław Plich, Ph.D. Dorota Milczarek

 

Objectives:

  • Studies on potato resistant to Phytophthora infestans
    • Studies on relationship between resistance to Phytophthora infestans and maturity.
    • Laboratory and field evaluations of resistance level in potato cultivars and breeding lines.

 

 

  • Combining a high level of resistance and agronomic traits.

  • Applying Sheffe-Caliński statistical model for evaluations of resistance stability in various environments.

  • Analysis and use of genetic diversity of table potato.

  • Understanding of the genetic basis of potato resistance to various pathotypes of Synchytrium endobioticum, the causal agent of potato wart disease.
    • Project aims of studying a new resistance source effective against broad spectrum of S. endobioticum pathotypes, which was identified in IHAR-PIB, Młochów Research Center in diploid potato hybrids and to understand the genetic determination of this resistance.
  • Methodological work on the use of molecular markers in potato breeding - the applying molecular markers in the selection potatoes resistant to nematodes (Globoderaspp.).


Multiplex PCR - simultaneous detection of markers of various genes conferring
resistance to potato cyst nematode

  • Study on a new source resistance to Globodera pallida found in Solanum gourlayi
  • Studies on potato with increased tuber concentration of important dietary compounds – analysis of genotype and environment influences on carotenoids accumulation in tubers.


Analysisof carotenoidsin potato tubers

  • Stability analysis of potato traits in different environments.
  • Examination of potato plants reaction to potato virus M - assessment of the impact of virus variants as well as genetic and environmental factors
design by VENTI