Młochów Division

Department of Potato Genetics and Parental Lines

Head of Department: Professor Waldemar Marczewski, DSc
Phone: (+48) 22 729 92 48 ext. 215
E-mail: w.marczewski@ihar.edu.pl

1. Monitoring genetic diversity of potato genotypes for potato breeding programs.
2. Genetic, genomic and proteomic studies of quantitative and qualitative traits in potato.
3. Identification of DNA markers useful to marker-assisted selection in potato breeding.
4. Diversity study of potato pathogen, mechanisms of potato resistance and pathogen-host interaction.
5. Development of diagnostic and selection methods useful in potato breeding programs and potato seed production.
6. Synthesis of diploid and tetraploid potato breeding lines for resistance to the most important pathogens, and quality traits.
7. Evaluation of potato cultivars and potato breeding materials for resistance to viruses.
8. Ecological aspects in potato cultivar production.
9. Physiological aspects of allelopathic interactions between potato and other plants.
10. Specialized trainings.

Publications (2017-2021)

1. Dees M. W., Lebecka R., Perminow J., Czajkowski R., Grupa A., Motyka A., Zoledowska S., Śliwka J., Lojkowska E., Brurberg M.B. 2017. Characterization of Dickeya and Pectobacterium strains obtained from diseased potato plants in different climatic conditions of Norway and Poland.  European Journal of Plant Pathology 148 (4): 839-851.
2. Flis B, Tatarowska B, Milczarek D, Plich J, 2017. Effect of location on starch content and tuber texture characteristics in potato breeding lines and cultivars. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science 67(5): 453 – 461.
3. Hara‑Skrzypiec A., Śliwka J., Jakuczun H., Zimnoch-Guzowska E. 2017. Quantitative trait loci for tuber blackspot bruise and enzymatic discoloration susceptibility in diploid potato. Molecular Genetics and Genomics DOI 10.1007/s00438-017-1387-0
4. Lamichhane J.R., Arseniuk E., Boonekamp P., Czembor J., Decroocq V., Enjalbert J., Finckl M., Korbin M., Kopel M., Kudsk P., Mesterhazy A., Sosnowska D., Zimnoch-Guzowska E., Messean A. 2018 „Advocating a need for suitable breeding approaches to boost Integrated Pest Management: A European perspective”. Pest Management Science DOI: 10.102/ps.4818
5. Milczarek D., Plich J., Tatarowska B., Flis B. 2017. Early selection of potato clones with resistance genes: the relationship between combined resistance and agronomical characteristics. Breeding Science 67(4):416-420
6. Przetakiewicz A., Milczarek D., Zimnoch-Guzowska E., Flis B. 2017 Improvement level of nematode resistance in potato facing changes in population of Globodera spp. The Journal of Animal and Plant Science 27(6): 1958-1962.
7. Smyda-Dajmund P., Śliwka J., Wasilewicz-Flis I., Jakuczun H., Zimnoch-Guzowska E. 2017. BC1 and F1 Progeny from Solanum × michoacanum (+) S. tuberosum Somatic Hybrids, Autofused 4× S. michoacanum and Cultivated Potato. American Journal of Potato Research 94 (4): 323–333
8. Stefańczyk E., Sobkowiak S., Brylińska M., Śliwka J. 2017. Expression of the potato late blight resistance gene Rpi-phu1 and Phytophthora infestans effectors in the compatible and incompatible interactions in potato. Phytopathology 107: 740-748.
9. Śliwka J., Brylińska M., Stefańczyk E., Jakuczun H., Wasilewicz-Flis I., Sołtys-Kalina D., Strzelczyk-Żyta D., Szajko K., Marczewski W. 2017. Quantitative trait loci affecting intensity of violet flower colour in potato. Euphytica: 213-254
10. Tatarowska B., Flis B., Plich J., Milczarek D., Zimnoch-Guzowska E. 2017. The suitability of different statistical measures to assess the stability of resistance to Phytophthora infestans (Mont.) de bary in potato cultivars in field conditions. Journal of Animal and Plant Sciences 27 (2): 510-516.
11. Yin Z, Xie F., Michalak K., Pawełkowicz M., Zhand B., Murawska Z., Lebecka R., Zimnoch-Guzowska E. 2017. Potato cultivar Etola exhibits hypersensitive resistance to PVYNTN and partial resistance to PVYZ-NTN and PVYN-Wi strains and strain-specific alterations of certain host miRNAs might correlate with symptom severity. Plant Pathology 66 (4): 539–550.
12. Szajko K., Strzelczyk-Żyta D., Marczeski W. 2017. Comparison of leaf proteomes of potato (Solanum tuberosum L.) genotypes with ER- and HR-mediated resistance to PVY infection. European Journal of Plant Pathoogy. doi 10.1007/s10658-017-1284-8
13. Lebecka R., Michalak K. 2017. Reakcja bulw wybranych odmian ziemniaka na porażenie przez wysoko wirulentny szczep bakterii Dickeya solani. Ziemniak Polski 3:18-23.
14. Lebecka R., Murawska Z. 2017. Reakcja roślin ziemniaka na porażenie przez bakterie Dickeya solani w teście szklarniowym. Ziemniak Polski 1: 10-14.
15. Milczarek D., Tatarowska B., Plich J., Przetakiewicz A., Flis B. 2017. Kumulacja odporności na mątwiki a poziom cech użytkowych ziemniaka. Ziemniak Polski 3: 15-18.
16. Plich J., Tatarowska B., Milczarek D., Flis B. 2017. Piramidyzacja genów odporności ziemniaka na Phytophthora infestans. Biuletyn IHAR 281: 69-76.
17. Zimnoch-Guzowska E. 2017. Znaczenie krajowej hodowli i nasiennictwa ziemniaka dla rozwoju sektora jego produkcji. Ziemniak Polski 3: 3-7.
18. Brylińska M., Sobkowiak S., Stefańczyk E., Śliwka J. 2018. Evaluation of PCR markers for Phytophthora infestans mating type determination. European Journal of Plant Pathology 152 (1): 33–44. 
19. Grupa A., Otulak K., Syller J. ”Serological, molecular and ultrastructural evidence for interference competition between isolates of Potato virus Y ”. Plant Pathology
20. Hara‑Skrzypiec A., Śliwka J., Jakuczun H., Zimnoch-Guzowska E. 2018. Quantitative trait loci for tuber blackspot bruise and enzymatic discoloration susceptibility in diploid potato. Molecular Genetics and Genomics 293(2): 331-342.
21. Hara-Skrzypiec A., Śliwka J., Jakuczun H., Zimnoch-Guzowska E. 2018. QTL for tuber morphology traits in diploid potato. Journal of Applied Genetics 59(2): 123-132.
22. Lamichhane J.R., Arseniuk E., Boonekamp P., Czembor J., Decroocq V., Enjalbert J., Finckl M., Korbin M., Kopel M., Kudsk P., Mesterhazy A., Sosnowska D., Zimnoch-Guzowska E., Messean A. 2018. Advocating a need for suitable breeding approaches to boost integrated pest management: a European perspective. Pest Management Science 74 (6): 1219-1277
23. Lebecka R., Flis B., Murawska Z. Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum and Dickeya solani. Journal of Phytopathology 166(9): 654-662
24. Plich J., Przetakiewicz J., Śliwka J., Flis B., Wasilewicz-Flis I., Jakuczun H., Zimnoch-Guzowska E. 2018. Novel gene Sen2 conferring broad-spectrum resistance to Synchytrium endobioticum mapped to potato chromosome XI. Theoretical and Applied Genetics 131(11): 2321-2331
25. Stefańczyk E., Brylińska M., Brurberg M.B., Naerstad R., Elameen A., Sobkowiak S., Śliwka J. 2018 Diversity of Avr-vnt1 and AvrSmira1 effector genes in Polish and Norwegian populations of Phytophthora infestans. Plant Pathology 67(8): 1792-1802
26. Szajko K., Strzelczyk-Żyta D., Marczewski W. 2018. Comparison of leaf proteomes of potato (Solanum tuberosum L.) genotypes with ER- and HR-mediated resistance to PVY infection. European Journal of Plant Pathology 150: 375-385.
27. Sołtys-Kalina D. 2017. Assesment of phytotoxic potential of potato genotypes. Plant Breeding and Seed Science 75:17-22.
28. Lebecka R. 2017. Isolation, identification and preservation of pectynolitic bacteria pathogenic. Plant Breeding and Seed Science 75:87-96.
29. Sobkowiak S., Śliwka J. 2017. Phytophthora infestans: isolation of pure cultures, storage and inoculum preparation. Plant Breeding and Seed Science 76: 9-15.
30. Stefańczyk E., Sobkowiak S. 2017. Isolation, identification and preservation of Fusarium spp. causing dry rot of potato tubers. Plant Breeding and Seed Science 76: 45-51.
31. Stefańczyk E. Assessment of potato tuber resistance against dry rot. 2017. Plant Breeding and Seed Science. 76: 54-56
32. Plich J., Tatarowska B. 2017. Field evaluation of potato resistance against Phytophthora infestans under natural infection pressure. Plant Breeding and Seed Science 76: 26-28 [złożone do systemu POL-on]
33. Hara-Skrzypiec A. 2017. Evaluation of tendency to blackspot brusing and enzymatic discoloration of potato tubers. Plant Breeding and Seed Science.75: 5-10
34. Plich J. 2017. Evaluation of the length of the vegetation period of potato. Plant Breeding and Seed Science 76: 65-67
35. Flis B. 2017. Selection of potato parental lines with complex resistances to potato pathogens and pests. Plant Breeding and Seed Science 76: 57-63
36. Tatarowska B., Milczarek D. 2017. Evaluation of potato cultivars and breeding lines for carotenoids content in tubers. Plant Breeding and Seed Science 75: 11-16
37. Yin Z., Michalak K. 2017. Diagnostics of the presence of viruses in the potato and soil. Plant Breeding and Seed Science.75: 37-55
38. Yin Z. 2017. Methods to study the PVY population in the potato. Plant Breeding and Seed Science 75: 71-76
39. Murawska Z., Dębski J., Szajko K., Lebecka R. 2017. Isolation of proteins from potato tubers. Plant Breeding and Seed Science 75: 23-27
40. Szajko K. 2017. Isolation and identification of proteins from potato leaves. Plant Breeding and Seed Science 75: 29-36
41. Michalak K., Chrzanowska M., Evaluation of resistance to potato virus Y of cultivars in pre-registration (statutory) trials. Plant Breeding and Seed Science 75: 63-70
42. Yin Z., Michalak K., Lebecka R. 2017. Collection of potato viral pathogens, its validation and maintenance. Plant Breeding and Seed Science 75: 57-62
43. Lebecka R. 2017. Screening for potato resistance to blackleg and soft rot. Plant Breeding and Seed Science 75: 97-104
44. Przetakiewicz A., Milczarek D. 2017. Evaluation of potato cultivars and breeding lines for resistance to Globodera rostochiensis and Globodera pallida. Plant Breeding and Seed Science. 76: 3-8
45. Przetakiewicz J., Plich J. 2017.  Assessment of potato resistance to Synchytrium endobioticum. Plant Breeding and Seed Science 76: 37-43
46. Brylińska M., Śliwka J. 2017. Laboratory assessment of potato resistance to Phytophthora infestans. Plant Breeding and Seed Science 76: 17-23
47. Smyda-Dajmund P. 2017. Cryopreservation of shoot tips and pollen of potato. Plant Breeding and Seed Science 76: 75-80
48. Smyda-Dajmund P.  2017. Virus elimination from in vitro potato plants. Plant Breeding and Seed Science 76: 81-85
49. Szajko K.,  Sołtys-Kalina D., Szarzynska B.,  Strzelczyk Żyta D., Szweykowska-Kulinska Z., Marczewski W. 2019.A comparative proteomic analysis of the PVY-induced hypersensitive response in leaves of potato (Solanum tuberosum L.) plants that differ in Ny-1 gene dosage. European Journal of Plant Pathology. 153(2):385-396.
50. Yin Z., Murawska Z., Xie F.,Pawełkowicz M.,Michalak K., Zhang B., Lebecka R. 2019. microRNA response in potato virus Y infected tobacco shows strain-specificity depending on host and symptom severity. Virus Research. 260:20-32.
51. Sołtys-Kalina D., Murawska Z., Strzelczyk-Żyta D., Wasilewicz-Flis I., Marczewski W.2019.  Phytotoxic potential of cultivated and wild potato species (Solanum sp.): role of glycoalkaloids, phenolics and flavonoids in phytotoxicity against mustard (Sinapis alba L.). Acta Physiologiae Plantarum. 158:41-55.
52. Lebecka R., Kistowski M.,Dębski J., Szajko K., Mirawska Z., Marczewski W.2019. Quantitative proteomic analysis of differentially expressed proteins in tubers of potato plants differing in resistance to Dickeya solani. Plant and Soil. 441:317-329.
53. Tatarowska B., Milczarek D., Wszelaczyńska E., Pobereżny J., Keutgen N., Keutgen A.J., Flis B. 2019. Carotenoids Variability of Potato Tubers in Relation to Genotype, Growing Location and Year. American Journal of Potato Research.96(5): 493-504
54. Szajko K., Yin Z., Marczewski W.2019. Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y. Potato Research.62(4):379-392.
55. Keutgen A., Wszelaczyńska E., Pobereżny J., Przewodowska A., Przewodowski W., Milczarek D., Tatarowska B, Flis B., Keutgen N. 2019. Antioxidant properties of potato tubers (Solanum tuberosum L.) as a consequence of genetic potential and growing conditions. Plos One 14(9) article:0222976
56. Lebecka R. I Czarniak Z. 2019. Laboratoryjna ocena porażenia bulw ziemniaka przez wysoko agresywne szczepy bakterii Dickeya solani i Pectobacterium carotovorum subsp.
    1.  
57. Grech-Baran M., Witek K., Szajko K., Witek A., Morgiewicz K., Wasilewicz-Flis I., Jakuczun H., Marczewski W., Jones J.D.G, Hennig J .2019.  Extreme resistance to             Potato virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor. Plant Biotechnology Journal 18:655–667
58. Keutgen A.J., Keutgen N., Wszczelaczyńska E., Pobereżny J., Milczarek D.,             Tatarowska B., Flis B., 2019. Evaluation of Photosynthetic and Yield Traits in Ten      Potato Clones and Cultivars Under Farming Conditions in Poland. Potato Research          62:1-21
59. Szajko K., Plich J., Przetakiewicz J., Sołtys-Kalina D., Marczewski W., 2020.Comparative proteomic analysis of resistant and susceptible potato cultivars during Synchrytium endobioticum infestation, Planta 251 article:4
60. Sołtys-Kalina D., Szajko K., Wasilewicz-Flis I., Mańkowski D., Marczewski W., Śliwka J., 2020. Quantitative trait loci for starch-corrected chip color after harvest,             cold storage and after reconditioning mapped in diploid potato. Molecular Genetics and Genomics 295: 209-219.
61. Plich J., Zimnoch-Guzowska E., Tatarowska B., Śliwka J. 2020. Quantitative trait loci       analysis of potato tuber greening.  Molecular Biology Reports 47:1713-1722.    
62. Smyda-Dajmund P., Śliwka J., Janiszewska M., Zimnoch-Guzowska E. 2020. Cytoplasmic diversity of potato relatives preserved at Plant Breeding and Acclimatization Institute in Poland. Molecular Biology Reports 47: 3929–3935.
63. Tatarowska B., Plich J., Milczarek D., Flis B. 2020. Temperature‐dependent resistance             to potato virus M in potato (Solanum tuberosum), Plant Pathology 69:1445-1452.   
64. Stefańczyk E., Plich J., Janiszewska M., Smyda-Dajmund P., Sobkowiak S., Śliwka J.             2020. Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and           Rpi-phu1 on di- and tetraploid levels. Molecular Breeding. 40:89.
65. Sołtys-Kalina D., Szajko K., Stefańczyk E., Smyda-Dajmund P., Śliwka J.,Marczewski W. 2020. eQTL mapping of the 12S globulin cruciferin gene PGCRURSE5 as a novel candidate associated with starch content in potato tubers. Scientific Reports. 10:17168.
66. Prodhomme C, Arkel G., Plich J., Tammes J., Rijk J., Eck H., Visser R., Vossen J., A Hitchhiker’s guide to the potato wart disease resistance galaxy.  Theoretical and Applied Genetics volume. 133: 3419–3439.
67. Plich J., Boguszewska-Mańkowska D., Marczewski W., Relations Between Photosynthetic Parameters and Drought-Induced Tuber Yield Decrease in Katahdin-Derived Potato Cultivars. 2020. Potato Research. 63: 463–477
68. Milczarek D., Tatarowska B., Plich J., Podlewska-Przetakiewicz A., Flis B., Solanum gourlayi — a Source of Cyst Nematode Resistance in Potato Breeding. 2020.             Potato Research. 63:589-595
69. Janiszewska M., Sobkowiak S., Stefańczyk E., Śliwka J., Population Structure of Phytophthora infestans from a Single Location in Poland Over a Long Period of Time in Context of Weather Conditions. 2021. Microbial Ecology. 81: 746–757 
70. Azil N., Stefańczyk E., Sobkowiak S., Chihat S., Boreghda H., Śliwka J., Identification and pathogenicity of Fusarium spp. associated with tuber dry rot and wilt of potato in Algeria.2021. Eur J Plant Pathol 159, 495–509.
71. Smyda-Dajmund P., Śliwka J., Villano C., Janiszewska M., Aversano R., Bednarek P.T., Carputo D., Zimnoch-Guzowska E., Analysis of Cytosine Methylation in Genomic DNA of Solanum × michoacanum (+) S. tuberosum Somatic Hybrids. 2021. Agronomy. 11.5:845
72. Zimnoch-Guzowska E., Flis B. Over 50 Years of Potato Parental Line Breeding Programme at the Plant Breeding and Acclimatization Institute in Poland. Potato Research. 64:743-760. 
73. Szajko K., Ciekot J., Wasilewicz-Flis I. Marczewski W., Sołtys-Kalina D. Transcriptional and proteomic insights into phytotoxic activity of interspecific potato hybrids with low glycoalkaloid contents. 2021. BMC Plant Biol. 21, 60
74. Lebecka R, Śliwka J, Grupa-Urbańska A, Szajko K, Marczewski W.  QTLs for potato tuber resistance to Dickeya solani are located on chromosomes II and IV. Plant Pathology. 2021, 70 (7) 1745-1756.
75. Lebecka, R., Wasilewicz-Flis I., Mańkowski D. Diploid potato germplasm with resistance to Dickeya solani. Potato Research. 64 (3) 375-385 
76. Yin Z., Xieb F., Michalak K., Zhangb B., Zimnoch-Guzowska E. Reference gene selection for miRNA and mRNA normalization in potato in response to potato virus Y. 2021. Molecular and Cellular Probes. 55:101691.
77. Milczarek D., Podlewska-Przetakiewicz A., Plich J, Tatarowska B., Flis B. The relations of broad nematode resistance to quality characteristics as a consequence of marker-assisted selection in potato breeding programs. 2021. Breeding Science 71(5) 609-614           0121
78. Yin Z., Chapter 1 - Host-encoded miRNAs in plant-virus interactions—What's new. 2021. Plant Virus-Host Interaction (Second Edition) Molecular Approaches and Viral Evolution Pages 3-43. https://doi.org/10.1016/B978-0-12-821629-3.00018-X